# Mouse Models and Metabolomics Tools to Investigate Alcohol Metabolism and Tissue Injury

> **NIH NIH R24** · YALE UNIVERSITY · 2021 · $511,168

## Abstract

ABSTRACT
The deleterious effects of alcohol are primarily mediated by its metabolic by-products. Ethanol metabolism by microsomal
and mitochondrial systems generates reactive oxygen and nitrogen species, and is associated with diminished glutathione
and antioxidant enzymatic activity, all of which promote oxidative stress. In addition, the accumulation of ethanol-derived
aldehydes and hydroxyethyl radicals serves to modify critical biological functions by forming adducts with proteins and DNA.
The availability of animal models in which ethanol metabolism or antioxidant mechanisms are genetically modified facilitates
investigation of the role these enzymes and oxidative stress play in diseases associated with ethanol consumption. The
goals of this application are to: A) maintain and develop unique transgenic knockouts that can be made available to the
larger research community for the investigation of the pathogenesis of alcohol abuse and the mechanisms underlying the
deleterious effects of alcohol, and B) offer metabolomics and tissue imaging mass spectrometry (IMS) expertise to facilitate
investigation of the biochemical consequences of alcohol exposure and pathogenesis. Our overarching aim is to provide
valuable transgenic animal models and metabolomics resources to the larger research community that will greatly enhance
our understanding of the mechanisms underlying alcohol-induced disease and the pathophysiological effects of acute and
chronic alcohol consumption. It is anticipated that our metabolomic platforms will offer state-of-the-art techniques to dissect
the molecular mechanisms of alcohol-induced tissue injury. Such knowledge will facilitate the development of more effective
treatments of alcohol abuse.

## Key facts

- **NIH application ID:** 10231089
- **Project number:** 5R24AA022057-10
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** VASILIS VASILIOU
- **Activity code:** R24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $511,168
- **Award type:** 5
- **Project period:** 2013-02-01 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10231089

## Citation

> US National Institutes of Health, RePORTER application 10231089, Mouse Models and Metabolomics Tools to Investigate Alcohol Metabolism and Tissue Injury (5R24AA022057-10). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10231089. Licensed CC0.

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