# Mouse Microsurgery Core

> **NIH NIH P01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2021 · $176,281

## Abstract

CORE B - SUMMARY/ABSTRACT
The Mouse Liver Microsurgery Core (Core B) will be responsible for conducting liver transplant surgery
procedures and post-transplant care in mice in this PPG initiative. The orthotopic liver transplantation (OLT)
procedures in mice are technically highly demanding, and the maintenance of surgical technical quality and
stability will be the key for the experimental success. Core B therefore will provide all the surgical assistance to
the investigators in conducting liver warm ischemia as well as ex vivo liver cold ischemia combined with OLT, as
outlined in Project I and Project II. The core will also coordinate experiments among the projects to maximize the
use of samples from transplants and minimize the number of animals for transplant surgery. In addition, extra
samples from transplants will be stored and maintained as the archive. If the Project requires additional surgical
procedures, Core B will also provide support for other surgical needs in the proposals. The laboratory has
developed the mouse model of extended hepatic cold storage and OLT a decade ago to study various aspects
of innate and adaptive immune responses in organ transplantation. Significant amounts of experience,
accumulated data, and available models will enable us to confidently conduct the complicated experiments in
this Project. The specific aims of Core B are as follows:
Aim 1. To provide OLT and local liver IRI surgery and post-transplant care in mice with quality and consistency.
The core will be responsible for conducting all the microsurgical OLT/non-transplant procedures and post-
transplant care in mice in Project 1 and 2. These procedures are technically demanding, and the Core will
assure consistent and reliable surgical outcomes in conducting experiments, as proposed in this PPG.
Aim 2. To coordinate experiments among projects and maximize the use of samples from liver transplants and
recipients. The Core will coordinate experiments between Project I and Project II to maximize the use of samples
from transplanted animals and thus to minimize the numbers and costs of animals for surgeries.
Aim 3. To store extra samples and maintain the archive. The Core will store and record surplus tissue samples
(liver grafts, host tissues, serum) and maintain them as an archive for potential future use.
Aim 4. To provide support for other surgical needs. Core B will provide support for additional surgical procedures
(e.g., portal vein injections; i.v. infusions), if needed.

## Key facts

- **NIH application ID:** 10232243
- **Project number:** 5P01AI120944-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** Haofeng Ji
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $176,281
- **Award type:** 5
- **Project period:** 2017-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10232243

## Citation

> US National Institutes of Health, RePORTER application 10232243, Mouse Microsurgery Core (5P01AI120944-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10232243. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*