# Single Cell and Immunoanalysis Core

> **NIH NIH P30** · BRIGHAM AND WOMEN'S HOSPITAL · 2021 · $141,056

## Abstract

Project Summary/Abstract: The Single Cell and Immunoanalysis Core
Research into human skin biology and inflammatory skin diseases has been slowed by the lack of transgenic
model systems and the limited samples that can be obtained from living patients. However, advanced analytic
techniques that provide maximal information from small samples have been developed that have the potential
to advance our understanding of human skin disease. This Core makes critical groundbreaking techniques
including single cell RNA sequencing (scRNA-seq) with the ability to batch samples, discover TCR genes and
tag cells with antibodies (CITE-seq), as well as single cell ATAC-seq, single cell imaging by flow cytometry,
cytometry by time of flight (CyTOF) and high throughput T cell receptor sequencing available to investigators at
any institution who wish to carry out human skin disease research. The Center also provides assistance with
experimental planning, key reagents and assistance with data analysis for each technique. For example, the
Center provide protocols that allow skin biopsies to be shipped from distant locations and viably cryopreserved
before scRNA-seq studies; this advance makes single cell profiling available to investigators to study samples
from clinical trials and from understudied skin diseases. The Center also provides CyTOF staining protocols,
validated panels of CyTOF antibodies, custom conjugation of CyTOF antibodies, advice on experimental
design and data analysis, and all services needed for outside investigators to send their cells to the Center for
CyTOF studies. The Center provides comprehensive support in the experimental design, DNA extraction
protocols, DNA sample preparation services, shipping, and data analysis assistance that will enable
researchers to successfully utilize high throughput TCR sequencing to profile skin immune responses and
track pathogenic T cell clones across multiple timepoints and tissues. We have included 36 research projects
from investigators who wish to utilize the services of this Core. In summary, this core provides access to single
cell approaches that have the potential to provide unprecedented insights into the biology of human skin
disease.

## Key facts

- **NIH application ID:** 10232690
- **Project number:** 2P30AR069625-06
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** JAMES A. LEDERER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $141,056
- **Award type:** 2
- **Project period:** 2016-07-19 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10232690

## Citation

> US National Institutes of Health, RePORTER application 10232690, Single Cell and Immunoanalysis Core (2P30AR069625-06). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10232690. Licensed CC0.

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