# Generation of Abi3 conditional knockout mouse model

> **NIH NIH R21** · INDIANA UNIVERSITY INDIANAPOLIS · 2021 · $431,765

## Abstract

Project Summary/Abstract
Recent human genetics studies of Alzheimer’s disease have revealed that many risk genes are primarily
expressed in microglia. Microglia promote clearance of amyloid β (Aβ) through phagocytosis and regulate brain’s
immunity by secreting cytokines and chemokines in the context of Alzheimer’s disease. Because there have
been numerous in vitro studies suggesting protective as well as detrimental effects of different types of microglia
in Alzheimer’s disease, how microglial Alzheimer’s disease risk genes regulate the function of these cells still
remain elusive. To this end, determining the functional consequences of these genetic risk factors in Alzheimer’s
disease, particularly in microglia, can provide invaluable insight into the pathobiology of the disease. In a recent
large scale genome-wide analysis study, a rare coding variant was identified in Abelson interactor family member
3 (ABI3) gene and this variant is associated with increased risk of late-onset Alzheimer’s disease (LOAD). ABI3
is highly expressed in multiple myeloid cell types in the brain, spleen, and blood. ABI3 is a component of WAVE2
complex, a complex that regulates actin cytoskeleton organization. This complex is involved in cytokinesis,
migration, endocytosis, and phagocytosis. Here, we propose to generate Abi3 conditional knock-out mouse
model. Generation of Abi3-floxed (Abi3fl/fl) mouse model will enable us and the wider research community to
dissect out the cell-type specific role of Abi3 under the homeostatic and pathological conditions.

## Key facts

- **NIH application ID:** 10233131
- **Project number:** 1R21AG072738-01
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** Jungsu Kim
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $431,765
- **Award type:** 1
- **Project period:** 2021-06-01 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10233131

## Citation

> US National Institutes of Health, RePORTER application 10233131, Generation of Abi3 conditional knockout mouse model (1R21AG072738-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10233131. Licensed CC0.

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