# Real-time In Vivo Visualization of the Molecular Processes in Choroidal Neovascularization

> **NIH NIH K08** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 2021 · $224,397

## Abstract

ABSTRACT
 Neovascularization plays a pivotal role in the leading causes of blindness in the developed world,
including wet age-related macular degeneration (AMD). At its early stage, wet AMD is characterized by
molecular changes. Later, choroidal neovascularization (CNV) develops, leading to subretinal hemorrhage,
scarring, and irreversible vision loss. Thus, detection of wet AMD at an earlier stage, before the hemorrhage
develops, can improve vision. This K08 research will develop and investigate a novel multimodal molecular
imaging system using photoacoustic microscopy (PAM), optical coherence tomography (OCT), and
fluorescence microscopy to detect wet AMD at an earlier stage than currently possible using molecular
contrast agents to visualize αvβ3 integrin in neovascularization. CNV will be localized and quantified to sub-10
micron resolution. This real-time, in vivo molecular information will allow for targeted treatment and precision
medicine tailored to each patient’s unique molecular expression.
 The central hypothesis is molecular imaging of αvβ3 integrin will be sensitive and specific in early
choroidal neovascularization and can thus be used as a biomarker in early detection of neovascularization in
macular degeneration. The objectives are to: 1) demonstrate the safety of photoacoustic microscopy; 2)
perform multimodal molecular imaging of αvβ3 integrin using PAM, OCT, and fluorescence microscopy; and 3)
demonstrate that molecular imaging of αvβ3 integrin allows for earlier detection of CNV in rabbit models. The
two specific aims of this study are 1) Test the prediction that photoacoustic microscopy (PAM) can safely
visualize the chorioretinal microvasculature, and 2) Quantify the extent that molecular imaging using gold
nanoparticles targeting αvβ3 integrin localize to CNV and enable earlier visualization of CNV.
 The long-term goals of this career development research plan is for the investigator to develop the skills
and expertise in high resolution, multimodal molecular ophthalmic imaging to understand the molecular
mechanisms leading to choroidal neovascularization. This will improve the care of patients through early
detection diagnosis, precision medicine, and improved understanding of fundamental biology. The world-
renowned mentors and advisors from the University of Michigan are leaders of their respective fields and are
fully commited to guiding the candidate's development into an independent investigator clinician scientist.

## Key facts

- **NIH application ID:** 10237996
- **Project number:** 5K08EY027458-04
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **Principal Investigator:** Yannis Mantas Paulus
- **Activity code:** K08 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $224,397
- **Award type:** 5
- **Project period:** 2018-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10237996

## Citation

> US National Institutes of Health, RePORTER application 10237996, Real-time In Vivo Visualization of the Molecular Processes in Choroidal Neovascularization (5K08EY027458-04). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10237996. Licensed CC0.

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