Systems analysis of the prostate cancer epigenome
Project Summary/Abstract Although androgen ablation is initially effective for advanced prostate cancer treatment, resistance ultimately develops through various mechanisms, causing patient morbidity. Systems analysis of the prostate cancer epigenome is a genome-scale approach to understanding the under-studied epigenetic events that characterize prostate cancer progression to the resistant state. This proposed work is an integral part of the NCI-funded research program U54 CA217297, which focuses on studying epigenetic mechanisms exploited by hormone- resistant cancer cells to acquire growth and invasion advantages. Dr. Qianben Wang’s laboratory has recently performed an integrative analysis of strand specific paired-end ChIP-exo (ChIP-ePENS) and MNase-seq data and discovered an epigenetic mechanism by which genomic and transcriptional regulation are controlled by precise changes to nucleosome positioning within transcription factor (TF) binding sites. This represents the latest in a