# Core D - Immunopathology

> **NIH NIH P01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2021 · $160,375

## Abstract

CORE SUMMARY
The overall goal of this PPG application is to develop a mechanistic understanding of the cellular and
molecular basis for PD1 and LAG3 interactions that regulate T cell function in autoimmunity, cancer and chronic
viral infections. Core D will provide sophisticated and relevant technologies and expertise to PPG investigators
for understanding how the interplay between PD1 and LAG3 affects cellular interactions
and relevant molecular pathways in intact experimental tissues from mice with autoimmune disease
(Project 1), cancer (Project 2) and chronic viral infection (Project 3). The Aims of this Core are: AIM 1: To
provide basic expertise and technologies for detailed immunopathologic tissue analysis; AIM 2: To apply high-
resolution quantitative, correlative immunopathology using highly-multiplexed cyclic immunofluorescence (t-
CyCIF); and AIM 3: To maintain an annotated biospecimen bank/archive for future studies. These data will
interface with data generated by Core C and PPG projects in order to develop protocols to visualize new
molecules, as well as metrics generated from conventional histology (e.g., qualitative assessment and grading
of lesion severity) and clinical parameters (e.g. survival and weight loss). Core D will interact extensively with
all three Projects, with Core A for oversight and administrative support, and Core C by developing new staining
protocols for cellular and histologic investigation of key genes and their products that Core C determines are
regulated by PD1 and LAG3.

## Key facts

- **NIH application ID:** 10239109
- **Project number:** 5P01AI108545-07
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** GEORGE F MURPHY
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $160,375
- **Award type:** 5
- **Project period:** 2015-05-15 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10239109

## Citation

> US National Institutes of Health, RePORTER application 10239109, Core D - Immunopathology (5P01AI108545-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10239109. Licensed CC0.

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