# Genetic Analysis and Manipulation Core (GAEC)

> **NIH NIH P50** · BOSTON CHILDREN'S HOSPITAL · 2021 · $295,957

## Abstract

ABSTRACT
The Genetic Analysis and Editing Core (GAEC) provides cost-effective, state-of-the-art molecular tools and
consulting/bioinformatic services to IDDRC investigators for 1). Sequencing DNA at all scales, 2). Development
of gene editing strategies for cellular and animal models of human neurodevelopmental disease, and 3).
Quantitative analyses of gene expression in normal and diseased cells and tissues. GAEC offers services
through its Genetic Analysis Group (GAG) and the Gene Editing Group (GEG). The GAG offers Sanger
sequencing to validate plasmid constructs, sequence PCR products and for all molecular experiments requiring
DNA sequencing, and a full suite of assays and tools for genomic-level analysis, starting with high throughput
DNA purification, quantification and quality control assays, analytical DNA testing including genotyping in low,
medium and high throughput formats, Sanger and next generation sequencing (NGS), structural variant
detection, and low, medium and high throughput gene expression studies including single cell transcriptomics.
Bioinformatic support is also provided. The GEG enables the development of animal models of human IDDs
utilizing the latest gene targeting approaches and specializes in gene editing of the mouse genome via
CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeat)/(CRISPR associated protein) which
enables generation of novel gene-edited mouse lines with point mutations, insertions or deletions in a single
step. A cryopreservation service is also offered as well as advice on editing strategies and genotyping.
Consolidation of these overlapping analytic and editing technologies within a single core facility serving
multiple investigators allows individual investigators access to specialized techniques and to use these to
identify IDDRC related mutations, model these in mice and cell lines, and measure changes in gene
expression in tissues or at a single cell level. The combination of two pre-existing cores into a single integrated
core brings their overlapping activities into better alignment and offers a more comprehensive and complete
service to IDDRC investigators.

## Key facts

- **NIH application ID:** 10239466
- **Project number:** 1P50HD105351-01
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **Principal Investigator:** CLIFFORD J WOOLF
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $295,957
- **Award type:** 1
- **Project period:** 2021-07-22 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10239466

## Citation

> US National Institutes of Health, RePORTER application 10239466, Genetic Analysis and Manipulation Core (GAEC) (1P50HD105351-01). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10239466. Licensed CC0.

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