# High-Throughput Platform for Thermodynamic Characterization of Biomolecular Interactions

> **NIH NIH R01** · PALO ALTO RESEARCH CENTER · 2021 · $543,306

## Abstract

In this project we plan to further develop our microfluidic droplet calorimetry platform to enable thermodynamic
characterization of a wide variety and large number of biomolecular interactions. Having shown proof of
concept of the technology, we plan to increase the sensitivity and throughput of the system to enable
characterization of a broad range of reactions. The aims of the project are to 1) achieve multiplexed high-
resolution temperature readout for individual droplets, 2) increase the throughput of the system via automation
of the fluidic handling and data analysis, and 3) demonstrate and validate the technique by performing
calorimetric measurements of a variety of test samples. Taken together, these improvements will allow for a
dramatic expansion of calorimetry’s capabilities and, further, make those capabilities more accessible to the
scientific community.

## Key facts

- **NIH application ID:** 10242119
- **Project number:** 5R01GM132324-03
- **Recipient organization:** PALO ALTO RESEARCH CENTER
- **Principal Investigator:** Joerg Martini
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $543,306
- **Award type:** 5
- **Project period:** 2019-09-20 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10242119

## Citation

> US National Institutes of Health, RePORTER application 10242119, High-Throughput Platform for Thermodynamic Characterization of Biomolecular Interactions (5R01GM132324-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10242119. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*