# Role of Host Protein Kinase C in Cryptosporidiosis

> **NIH NIH R01** · UNIVERSITY OF VIRGINIA · 2021 · $638,364

## Abstract

PROJECT SUMMARY
Impact: This proposal addresses the pressing need for effective treatments for cryptosporidiosis in infants. The
therapeutic target is human protein kinase C (PKCα), which we recently discovered as a host susceptibility gene
for cryptosporidiosis. Successful completion of this work will broadly advance the field of host-targeted therapy
by taking a hit from a forward-genetic screen through mechanistic validation during disease.
Significance: Cryptosporidiosis is one of the top 5 causes of diarrhea in children in low- and middle-income
countries. There is no vaccine and the drugs are extremely limited with none approved for infants. Thus, there
is an urgent need for effective therapies, particularly for children. This proposal sets a precedent for developing
host targets for microorganisms, an area of high significance in the face of emerging antimicrobial resistance.
Innovation: A genome-wide scan for infectious disease susceptibility in infants from low-resource countries had
never been done until it was pioneered by our investigator team. The proposal integrates human genetics and
parasite cell biology to develop the discovery of PKCα into a target for host directed therapy. Our previous work
identified novel host factors that could be blocked to prevent cell-killing by Entamoeba parasites. We propose to
expand this innovative approach to Cryptosporidium. This strategy is fundamentally different from traditional
parasite-targeted drugs and is based on preliminary data that PKCα is a novel host susceptibility factor for
cryptosporidiosis.
Approach: We have discovered that human PKCα is a key component of susceptibility to cryptosporidiosis in
infants using a genome wide scan for disease-associated loci. We hypothesize that PKCα is required for
intracellular infection by Cryptosporidium and could be targeted to cure cryptosporidiosis. We will define the role
of PKCα in a multi-disciplinary study that integrates an analysis of PKCα during natural infection in infants
(Aim 1), targeted sequencing of the PKCα locus to identify the causal SNPs and mechanism of action (Aim 2),
and an analysis of host PKCα during in vitro and in vivo infection studies in combination with testing of FDA-
approved PKCα antagonists (Aim 3). Successful completion of this R01 will result in an Investigational New
Drug Application for a host-targeted PKC drug for cryptosporidiosis.
Environment: The Investigators bring strong and complementary skills to accomplish this work. Dr. Marie (PI)
is an expert in host and parasite cell and molecular biology. Dr. Duggal leads an internationally regarded program
in human genetic susceptibility to infection and Dr. Haque brings extensive experience in field studies in children
in Bangladesh. Importantly this team is highly collaborative as evidenced by their past discoveries and co-
publications. The team brings together three internationally recognized global health centers: The University of
Virginia (Dr. Marie), the I...

## Key facts

- **NIH application ID:** 10242203
- **Project number:** 5R01AI148518-02
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** Chelsea S. Marie
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $638,364
- **Award type:** 5
- **Project period:** 2020-08-20 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10242203

## Citation

> US National Institutes of Health, RePORTER application 10242203, Role of Host Protein Kinase C in Cryptosporidiosis (5R01AI148518-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10242203. Licensed CC0.

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