# Cryogenic Electron Microscopy Core

> **NIH NIH U54** · SEATTLE CHILDREN'S HOSPITAL · 2021 · $509,030

## Abstract

ABSTRACT
Single-particle cryogenic-electron microscopy (cryo-EM) has revolutionized structural biology through its ability
to tackle and elucidate structures of numerous macromolecular complexes that have previously been
impervious to structural studies. Over the last several years, it has become increasingly clear that some
inherent advantages in cryo-EM make it more suitable to structural studies of certain macromolecules,
especially those that are large, dynamic, difficult to purify, and/or poorly biochemically behaved. Cryo-EM has
therefore quickly become a mainstay in structural biology. Core 1 will employ the latest generation tools in
single-particle cryo-EM for the purpose of specifically addressing structural questions relevant to the HIVE P50
center. The tools will be used to solve structures of macromolecular complexes that are involved in the HIV
lifecycle, including viral replication, integration, assembly, and maturation, and will thus expand the evolving
structural knowledge underlying the molecular processes associated with HIV infection. This in turn will broadly
facilitate the improvement of antiretroviral therapies and lead to a better understanding of viral resistance. The
Cryo-EM core will: provide basic services that will evaluate sample quality and feasibility of high-resolution
studies, a service that will be available to all HIVE members; provide advanced expertise in structural biology
to jointly drive high-resolution structure determination in collaboration with several key investigators; apply
ongoing or novel technical developments to specific projects on a per-need basis. Core 1 will thus be highly
interactive within, and an integral part of, the HIVE center and will coordinate with all its investigators to
different extents.

## Key facts

- **NIH application ID:** 10242902
- **Project number:** 5U54AI150472-11
- **Recipient organization:** SEATTLE CHILDREN'S HOSPITAL
- **Principal Investigator:** Dmitry Lyumkis
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $509,030
- **Award type:** 5
- **Project period:** 2012-09-01 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10242902

## Citation

> US National Institutes of Health, RePORTER application 10242902, Cryogenic Electron Microscopy Core (5U54AI150472-11). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10242902. Licensed CC0.

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