# Tissue Culture and Hybridoma Core

> **NIH NIH P30** · CASE WESTERN RESERVE UNIVERSITY · 2021 · $193,649

## Abstract

The purpose of the Tissue Culture and Hybridoma Module is to enhance the capabilities of individual
investigators, whose vision-related research involves the use of cell cultures and monoclonal antibodies. The
Module is operated and supervised by highly experienced personnel and provides infrastructure for the
following Specific Aims: 1) to assist investigators in isolation of primary cells from ocular tissues; 2) to maintain
and passage established cell lines; 3) to perform large scale amplification of cell lines; 4) to conduct transient
cell transfection; 5) to generate stably transfected cell lines; 6) to provide strategic advice to and training of
laboratory personnel in planning of cell culture experiments and/or hybridoma production; 7) to produce,
isolate, and store hybridomas; 8) to purify monoclonal antibodies; 9) to perform mycoplasma testing; and 10) to
perform cryogenic preservation and storage of cells. The Module helps VSRC investigators to generate unique
reagents, to control all phases of their cell culture experiments and monoclonal antibody production, and
ultimately to conduct cutting-edge vision-related research in a cost-efficient manner.

## Key facts

- **NIH application ID:** 10244970
- **Project number:** 5P30EY011373-25
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** Robert Gerd Salomon
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $193,649
- **Award type:** 5
- **Project period:** 1997-04-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10244970

## Citation

> US National Institutes of Health, RePORTER application 10244970, Tissue Culture and Hybridoma Core (5P30EY011373-25). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10244970. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*