# Genome Sequencing

> **NIH NIH P20** · UNIVERSITY OF KANSAS LAWRENCE · 2021 · $210,185

## Abstract

PROJECT SUMMARY
 Next generation sequencing is now a central tool in the analysis of signaling pathways important in
development and disease. The Genome Sequencing Core (GSC) is aimed at providing researchers at the
University of Kansas and the state of Kansas with next-generation sequencing technologies as well as
expertise in experimental design and analysis of sequence data. Projects in the GSC include whole genome
assembly, genome re-sequencing for identification of mutations important in development and disease,
transcriptome analysis (RNA seq), variant mapping and genotyping, and identification of transcription factor
interaction sites using chromatin immunoprecipitation combined with DNA sequencing (ChIP seq). The GSC
houses an Illumina Hiseq 2500 sequencer and a MiSeq sequencer with dedicated processor and storage
space to run the instrument.
 This core facility has enhanced the genomics infrastructure already at KU, in the KU Genomics Center and
the KU DNA Sequencing Facility, which provides standard, first generation sequencing. Together, the three
cores allow for a thorough, integrated dissection of disease pathways using novel fluorescence probes to
define a cellular process, to find mutations affecting that cellular process, and to define genes involved in the
cellular process. The novel approach of the CMADP is to combine the enabling technologies of fluorescent
probe synthetic chemistry and next generation sequencing to set up a pipeline for target discovery in disease
pathways.
 Next generation sequencing is an enabling technology in that it has the power to allow investigators from
many different disciplines to ask new questions in their research areas of interest. The GSC enables genomics
research at the University of Kansas by eliminating the barriers of cost and communication involved with using
off-campus facilities. Interest in next generation sequencing at the University of Kansas is high, and includes
investigators in the Pharmacy School, the Department of Molecular Biosciences, and the Department of
Ecology and Evolutionary Biology. Use of the GSC will be by Projects in the COBRE Center for Molecular
Analysis of Disease Pathways, as well as by researchers across the State of Kansas and in many different
disciplines.

## Key facts

- **NIH application ID:** 10245046
- **Project number:** 5P20GM103638-10
- **Recipient organization:** UNIVERSITY OF KANSAS LAWRENCE
- **Principal Investigator:** Erik A Lundquist
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $210,185
- **Award type:** 5
- **Project period:** 2012-07-15 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10245046

## Citation

> US National Institutes of Health, RePORTER application 10245046, Genome Sequencing (5P20GM103638-10). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10245046. Licensed CC0.

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