# Genetically Modified Mouse Models

> **NIH NIH P30** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2021 · $312,479

## Abstract

GENETICALLY MODIFIED MOUSE MODEL SHARED RESOURCE: PROJECT SUMMARY 
Construction of transgenic and gene-targeted mice is an essential technology for cancer research, and the 
Herbert Irving Comprehensive Cancer Center (HICCC) has a long history of leadership in this field. Directed 
by Franklin Costantini, PhD, a pioneer in transgenic mouse technology, the Genetically Modified Mouse Model 
Shared Resource (GMMMSR) makes transgenic and gene targeting technologies accessible to HICCC 
members, generating hundreds of new genetically modified mouse strains. Services include: (1) generation 
of conventional and large construct (BAC) transgenics, (2) embryonic stem cell targeting and generation of 
knockout (KO) and knock-in mice, (3) generation of conditional KO mice, and (4) generation of CRISPR- 
based KO and knock-in strains.These comprehensive services are broadly used by HICCC members for the 
in vivo functional characterization of oncogenes and tumor supressors. During the current project period 
(2014-2019), GMMMSR incorporated CRISPR genome editing services to generate mouse lines with 
deletions, point mutations, and more complicated genetic manipulations such as exon swapping and reporter 
knock-in. The diversity of services provided and the unique technical expertise, easy accessibility and high 
cost-effectiveness of the GMMMSR make it the provider of choice for generation of genetically manipulated 
mouse models by HICCC members. The GMMMSR is invariably ranked as one of the most highly valued 
resources at Columbia University Irving Medical Center in annual users’ surveys. The GMMSR provides 
services that are not available from commercial sources (e.g., recombinant BAC transgenics) and not easily 
accessible at other sites, and that are of central importance to the research of numerous HICCC members. 
The GMMMSR has the capability to engineer novel mouse lines in about 10 months; and CRISPR-based 
gene targeting services can deliver a new genetically modified line in about three months. In addition, mouse 
lines generated by GMMMSR are transferred to HICCC members without a lengthy quarantine process, 
further accelerating and empowering HICCC member research. In addition to these advanced transgenic and 
gene targeting services, the GMMMSR offers investigators training, advice and assistance in the design of 
transgenes, gene-targeting vectors and CRISPR gene editing, mouse breeding, and the interpretation of 
experimental results. The GMMMSR will continue to incorporate novel technologies, to provide state-of-the- 
art transgenic and gene-editing services, and to promote high value and accessibility through service and 
consulting, with the goal of empowering the Research Programs of HICCC members. Over the current project 
period, the capabilities of the GMMMSR were utilized by 39 HICCC members, supported key data and insights 
for 28 HICCC member peer-reviewed publications, including15 papers with impact factor >10, of which nine 
ar...

## Key facts

- **NIH application ID:** 10245182
- **Project number:** 5P30CA013696-46
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Jianwen Que
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $312,479
- **Award type:** 5
- **Project period:** 1997-07-04 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10245182

## Citation

> US National Institutes of Health, RePORTER application 10245182, Genetically Modified Mouse Models (5P30CA013696-46). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10245182. Licensed CC0.

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