# Histopathology and Stone Analysis

> **NIH NIH P01** · UNIVERSITY OF CHICAGO · 2021 · $348,054

## Abstract

Project Summary 
Core B will conduct all the morphological and pathology analyses for Projects 1, 2, and 3. Papillary scoring is 
an essential part of the PPG, and Core B will analyze all surgical recordings from Projects 1 and 2 to quantify 
plaque and plug areas on all papillae and to ensure that papillary scoring across the projects is consistent. 
Human tissues will be collected by Project 2 from carefully categorized stone patients, and Core B will 
perform light and electron microscopy (LM and EM) using specialized stains on the biopsy material, and 
using micro- infrared spectroscopy (FT-IR) and Raman methods to identify mineral in the tissue. Core B will 
also prepare the frozen sections to be used by Project 3 for laser micro-dissection, along with 
immunostained sections to verify the biopsy content. All stones collected in Projects 1 and 2 will be analyzed 
by micro CT and conventional FT-IR to provide detailed information on stone type and percent mineral 
content. Core B will also perform atomic absorption spectrophotometric analysis of lithium to determine distal 
delivery of solutes in study patients (Project 1), and of calcium in tissue specimens (Project 3).

## Key facts

- **NIH application ID:** 10246875
- **Project number:** 5P01DK056788-20
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** JAMES CALDWELL WILLIAMS
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $348,054
- **Award type:** 5
- **Project period:** 2000-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10246875

## Citation

> US National Institutes of Health, RePORTER application 10246875, Histopathology and Stone Analysis (5P01DK056788-20). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10246875. Licensed CC0.

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