# Advanced TRPA1 Inhibitor for the Treatment of Chlorine Inhalation Injury

> **NIH NIH U01** · DUKE UNIVERSITY · 2021 · $784,984

## Abstract

Summary
 Chlorine gas has been used as a terrorist weapon, in warfare and has injured many Americans in
transportation or industrial accidents. Despite its devastating effects, no mechanism-based treatment has been
developed. In this application, we hypothesize that targeting the TRPA1 ion channel post-exposure will
ameliorate the acute pulmonary, cardiovascular and neurological effects of chlorine, leading to decreased
morbidity and improved recovery. TRPA1 is a chemical irritant receptor eliciting pain, edema, vasodilation,
cardiac arrhythmia, inflammation and leukocyte infiltration. Our preliminary studies in mice show that TRPA1
inhibitors, when administered post-chlorine exposure, prevent the chlorine-induced decline of blood
oxygenation, improve pulmonary function and mitigate inflammation.
 Here, we propose to test the efficacy of a 3rd generation TRPA1 inhibitor, found to block mouse, human
and porcine TRPA1, in mouse and pig models of chlorine inhalation injury, with the goal to develop this
compound as a future human countermeasure. The following aims are proposed: Aim 1: Screen potential
therapeutic effects of a 3rd generation TRPA1 inhibitor in mouse models of Cl2 gas inhalation injury. Aim 2:
Determine the pharmacokinetic and toxicological properties of TRPA1 inhibitor in pigs. Aim 3: Test the TRPA1
inhibitor in a pig model of chlorine gas inhalation injury

## Key facts

- **NIH application ID:** 10247523
- **Project number:** 5U01ES030672-03
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Satyanarayana Achanta
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $784,984
- **Award type:** 5
- **Project period:** 2019-09-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10247523

## Citation

> US National Institutes of Health, RePORTER application 10247523, Advanced TRPA1 Inhibitor for the Treatment of Chlorine Inhalation Injury (5U01ES030672-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10247523. Licensed CC0.

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