# Multiomic Signatures of Microbial Metabolites Following Prebiotic Fiber Supplementation

> **NIH NIH R01** · STANFORD UNIVERSITY · 2021 · $392,500

## Abstract

ABSTRACT
Phenolics-rich prebiotic fiber has been correlated with a number of positive health outcomes, however their
bioavailability is typically low. A large portion the gut microbiota metabolize these fibers releasing a range of
phenolic acids, which are believed to be principle bioactive components driving reduction in disease risk.
Despite this, little evidence exists linking specific gut bacteria with the metabolites they produce and the
downstream biological effects that these compounds exert. We therefore propose to assemble a cohort of
individuals that will receive prebiotic supplementation, during comprehensive, longitudinal characterization
of the microbiota and host changes with multiple omics assays (Aim 1). We will then integrate these multiomic
data (Aim 2), generating unique biological signatures that define the role microbial metabolites from specific
bacteria play in host biological activity. We will then isolate the microbial strains and metabolites associated
with host biological activity, validate their function in gnotobiotic mice and synthesize the newly identified
microbial molecules in vitro and demonstrate their structure, biological properties and applications (Aim 3).
Through this study we expect to gain a detailed and clear understanding of the physiological changes, at the
mechanistic level, that occur in the microbiome and host in response to dietary supplementation with prebiotic
fiber.

## Key facts

- **NIH application ID:** 10248323
- **Project number:** 5R01AT010232-04
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** MICHAEL P. SNYDER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $392,500
- **Award type:** 5
- **Project period:** 2018-09-20 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10248323

## Citation

> US National Institutes of Health, RePORTER application 10248323, Multiomic Signatures of Microbial Metabolites Following Prebiotic Fiber Supplementation (5R01AT010232-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10248323. Licensed CC0.

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