# Core A: High-Throughput Screening Core

> **NIH NIH P30** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $183,094

## Abstract

ABSTRACT
The High-Throughput Screening (HTS) Core (Core A) carries out target- and phenotype-based screens of
small-molecule libraries. The HTS core will directly support the activities of more than ten CF-related screening
projects, including correctors and potentiators for rare CFTR mutations such as N1303K, W1282X and
c.3700A>G, as well as several additional ion transport targets, including SLC26A gene family members, that
are relevant to pulmonary and non-pulmonary CF indications. The instrumentation available for screening
includes a fully automated screening platform with liquid handling and two plate readers, and multiple separate
plate readers some with stackers. Plate-readers provide fluorescence, polarization, absorbance, and
luminescence readout. Functions of Core A include compound storage and handling, assay design and
validation, assay execution, and data analysis. Core A resources also include HPLC, LC/MS, and NMR
instrumentation for compound quality control. Core A also carries out optimization of validated initial `hits'
(active compounds) by screening commercially available analogs. Further `hit-to-lead' development is done on
individual projects in consultation with the Chemistry Core (Core C). Having operated Core A for the past 14
years, the Core has established practical procedures for efficient and cost-effective compound screening,
quality control, and hit-to-lead optimization.

## Key facts

- **NIH application ID:** 10248485
- **Project number:** 5P30DK072517-17
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** ALAN S VERKMAN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $183,094
- **Award type:** 5
- **Project period:** 2005-08-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10248485

## Citation

> US National Institutes of Health, RePORTER application 10248485, Core A: High-Throughput Screening Core (5P30DK072517-17). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10248485. Licensed CC0.

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