# Center for Developmental Mapping of Heart and Bone Tissues

> **NIH NIH U54** · CHILDREN'S HOSP OF PHILADELPHIA · 2021 · $500,000

## Abstract

PROJECT SUMMARY - OVERALL
Both human bone and heart present remarkable anatomical, cellular and functional heterogeneity, with
specialized cellular structures performing distinct yet essential physiological functions. A significant gap of
knowledge is that different cells' molecular signature, spatial distribution and interactions, and functional state
remain little understood for both organs. Although spatially separated, growing evidence suggests that a bone-
heart communication axis plays a critical role in normal development and pathogenesis of these two organs
and possibly other organs. Joint analysis of the two organs could reveal novel insights into the
microenvironment organization and signaling pathways underlying the inter-organ communication. We
therefore propose the in-depth characterization of these two organs which, together, account for a large
fraction of human disease burden. We will map molecular and cellular changes in the tissue over the course of
human lifespan using comprehensive multi-dimensional single-cell and imaging technologies. The final product
will impact research of these two organs in the following manner: 1) Organ Atlases: spatially resolved atlases
will provide a highly user friendly, publicly available, searchable database of the most comprehensive multi-
omic, single cell analysis of the two organs. Molecular data will be richly annotated with additional clinical and
epidemiological data. 2) Computational methods: in addition to the data, the critical computational tools and
pipelines developed in this project will be available to the research community. These include methods and
pipelines for processing multi-omics and imaging data, inference of cell-specific regulatory and signaling
pathways, correlation of mesoscale imaging and molecular imaging features, as well as database algorithms
for the query, exploration and visualization of highly complex data. 3) Access to biospecimens for follow-up
studies: biospecimens collected in this project will be banked and made available to the biomedical research
community. These include freshly frozen and fixed specimens and tissue sections. In summary, the proposed
project will broadly impact the entire research community and jumpstart basic-science and medical discoveries
based on a sophisticated understanding of the key molecular circuits underlying the development and aging of
these two organs.

## Key facts

- **NIH application ID:** 10251350
- **Project number:** 5U54HL156090-02
- **Recipient organization:** CHILDREN'S HOSP OF PHILADELPHIA
- **Principal Investigator:** KATHRIN M BERNT
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $500,000
- **Award type:** 5
- **Project period:** 2020-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10251350

## Citation

> US National Institutes of Health, RePORTER application 10251350, Center for Developmental Mapping of Heart and Bone Tissues (5U54HL156090-02). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10251350. Licensed CC0.

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