# Dynamic Spatiotemporal Analysis of Immune Cells in the Tumor Microenvironment

> **NIH NIH F32** · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · 2020 · $30,613

## Abstract

Abstract
In our study, `Dynamic Spatiotemporal Analysis of Immune Cells in the Tumor Environment' we propose to
systemically characterize the spatiotemporal dynamics of immune cells in the tumor microenvironment using a
novel imaging technique developed by our collaborators at Stanford University. This technique enables the
acquisition of multi-dimensional data by high-throughput visualization of 20-plus proteins from a single tumor
section (CO-Detection by indexing). Using this comprehensive imaging approach we will longitudinally study
how the organization of immune cells, including tumor-associated macrophages, dendritic cells, and T cells
changes over the course of lung tumor development; specifically, by characterizing the dynamics of immune
cell remodeling, analyzing expression of activation/suppressive receptors, chemokine receptors and cytokine
production. The fundamental principles of immunology have taught us that the organization of immune cells
within the tissue is fundamental to their function. Thus, in this proposal we will test the hypothesis that over the
course of tumor growth the immunosuppressive tumor microenvironment abrogates the directed
reorganization, cell-to-cell interactions and communication of immune cells, necessary for the eradication of
tumor cells. These studies will shed important new light on how the immunouppressive tumor
microenvironment alters the cellular mechanism that regulate geographical orientation and motile behavior of
immune cells and findings from our study will ultimately aid us in identifying new effective targets for cancer
immunotherapy.

## Key facts

- **NIH application ID:** 10251841
- **Project number:** 5F32CA232666-03
- **Recipient organization:** NEW YORK UNIVERSITY SCHOOL OF MEDICINE
- **Principal Investigator:** Oriana Perez
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $30,613
- **Award type:** 5
- **Project period:** 2018-08-01 → 2021-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10251841

## Citation

> US National Institutes of Health, RePORTER application 10251841, Dynamic Spatiotemporal Analysis of Immune Cells in the Tumor Microenvironment (5F32CA232666-03). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10251841. Licensed CC0.

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