# Multistage LC-MSn for automated glycan isomer assignment of glycopeptides

> **NIH NIH R41** · PROTEIN METRICS, LLC · 2021 · $256,570

## Abstract

N- and O-linked glycosylation are some of the most common, abundant, and biologically
important posttranslational modifications of proteins, yet they are also some of the most difficult
to study. The dominant analytical platform for the study of glycosylation is mass spectrometry,
yet this platform on its own is incomplete, because it cannot elucidate glycan topology and
linkage stereochemistry. This project will advance the use of mass spectrometry for glycan
structure determination, especially for the case of O-linked glycans, by studying the ion
propensities of various structural motifs in multi-stage ion trap mass spectrometry, and by
building new software for structure inference from such mass spectra.

## Key facts

- **NIH application ID:** 10255170
- **Project number:** 1R41GM142363-01
- **Recipient organization:** PROTEIN METRICS, LLC
- **Principal Investigator:** Miklos Guttman
- **Activity code:** R41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $256,570
- **Award type:** 1
- **Project period:** 2021-04-16 → 2022-10-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10255170

## Citation

> US National Institutes of Health, RePORTER application 10255170, Multistage LC-MSn for automated glycan isomer assignment of glycopeptides (1R41GM142363-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10255170. Licensed CC0.

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