# Digital Melt Curve Analysis Platform for Longitudinal Cancer Patient Monitoring

> **NIH NIH R44** · COMBINATI, INC. · 2021 · $772,720

## Abstract

Abstract
 Building on its commercially available Absolute Q digital PCR platform, during the 24-month Phase 2
SBIR project, Combinati will complete the development of the first Digital Melt Curve Analysis (DMCA)
platform to the market to further advance the adoption of digital genomics for highly accurate, sensitive
and reproducible nucleic acid quantification for longitudinal patient monitoring. To provide the “whole
product” solution and prove the function, we will demonstrate the DMCA platform with Luminex’s 11-
plex ESR1 (Estrogen Receptor 1) assay and conduct Beta testing at Dana Farber Cancer Institute:
1. Three beta instruments and the analysis software capable of digital melt curve analysis.
2. Complete dMCA validation internally with commercially available melt calibration kits.
3. Demonstrate <0.1% Mutation Allele Frequency of 11 cell-free DNA targets using Luminex
 Corporation’s discrete melt assay.
4. Beta testing at Dana Farber Cancer Institute

## Key facts

- **NIH application ID:** 10256226
- **Project number:** 1R44CA261523-01
- **Recipient organization:** COMBINATI, INC.
- **Principal Investigator:** Paul Ju-Sung Hung
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $772,720
- **Award type:** 1
- **Project period:** 2021-04-01 → 2021-05-11

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10256226

## Citation

> US National Institutes of Health, RePORTER application 10256226, Digital Melt Curve Analysis Platform for Longitudinal Cancer Patient Monitoring (1R44CA261523-01). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10256226. Licensed CC0.

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