PROJECT SUMMARY/ABSTRACT The University of Pennsylvania Prevention of Lower Urinary Tract Systems Clinical Center (PENN+PLUS CC) is a diverse, experienced, committed and invaluable member of the Prevention of Lower Urinary Tract Symptoms (PLUS) Network, presenting an exceptional team of interdisciplinary investigators bringing expertise in adolescent and women’s pelvic health care, health behavior and prevention science, instrument development, and epidemiology. We are distinguished by our focus on implementation science and our unique location at the intersection of urban and rural areas and benefit from the significant and unique wealth of resources of UPenn. In Phase 1, the PENN+PLUS investigators made significant contributions to all PLUS publications and presentations during Phase 1. This includes 11 articles published or in press, 6 submitted manuscripts, and 26 refereed national and international presentations For Phase 2, to bolster the strength of our engagement with the community, we have partnered with the Penn Community Engagement Core, which will develop a realistic plan for inclusion of community partners, and have added new team members as Co-investigators, Drs Heather Klusaritz and Terri Lipman, who have expertise in community-based participatory research methods. We propose specific aims for Phase 2 efforts that address all of the needs stipulated in the RFA, including support for a nationally representative population-based large cohort study of bladder health (Aim 1), a project to develop and validate a novel tool for evaluating bladder self-care among adolescent girls and women (Aim 2), and pilot testing of a community-based bladder health promotion program (Aim 3).