# Defining a transcriptional periodic table of the human brain using reprogramming

> **NIH NIH DP1** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2020 · $1,354,500

## Abstract

The human brain contains diverse neural cell types that are differentially responsible for distinct aspects of
human behavior, cognition and neurologic disease. Advances in DNA sequencing are providing new insight
into human specific neurobiology by providing lists of genes that increase risk for disease or correlate with
behaviors and cognitive properties that differ between individuals. However, the lack of available human
neuronal subtypes and our limited understanding of which genes are expressed in different human neurons are
major barriers to exploiting these growing genomic resources. One way to overcome this barrier is to use direct
reprogramming to produce induced neuronal cells in vitro, by transiently expressing transcription factors (TFs)
in fibroblasts. Direct reprogramming produces induced neurons that share many features with endogenous
neurons, including characteristic morphologies, ligand-evoked synaptic responses and characteristic patterns
of gene expression. Reprogramming therefore offers a new tool to identify transcriptional circuits that establish
distinct features of neuronal identity. We, and others, have used candidate gene approaches to engineer
induced neurons that functionally mimic well-characterized neuronal subtypes, such as the peripheral sensory
neurons that detect pain and itch produced recently by my laboratory. These studies led us to hypothesize that
direct reprogramming engages conserved transcriptional circuits similar to those that actively maintain
neuronal subtype identity in endogenous neurons. This hypothesis predicts that it should be possible to identify
multiple TF combinations that induce distinct features of different neuronal subtypes. To test this hypothesis
and establish a systematic method to produce and classify human neuronal subtypes, we will conduct an
unbiased screen for new TF combinations that can induce human neuronal subtypes in vitro. We will then
characterize the induced neurons transcriptionally, morphologically and functionally. We are well suited to
perform this study because we recently conducted a pilot screen of ~600 TF pairs and identified more than 70
new pairs that produce candidate induced neurons from mouse fibroblasts. Gene expression profiling and
functional analyses of these cells confirm that they exhibit extensive subtype diversity. Therefore, by using
unbiased screens to define sets of human TFs that can induce neuronal identity in fibroblasts, we will identify
new methods to produce human neuronal cell types with defined functional properties in vitro and establish a
database of transcriptional programs and cellular properties that emerge from transient expression of different
sets of TFs. These studies will have impact on our understanding of the basic biology of human neuronal
diversity and will provide conceptual and practical tools to enable neuroscience researchers to produce diverse
subtypes of human induced neurons for research and translational applications...

## Key facts

- **NIH application ID:** 10263622
- **Project number:** 7DP1AG055944-06
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Kristin Kay Baldwin
- **Activity code:** DP1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $1,354,500
- **Award type:** 7
- **Project period:** 2020-09-15 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10263622

## Citation

> US National Institutes of Health, RePORTER application 10263622, Defining a transcriptional periodic table of the human brain using reprogramming (7DP1AG055944-06). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10263622. Licensed CC0.

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