# Polarized Exocytosis: Rabs, Tethers, and SNAREs

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 2021 · $445,663

## Abstract

Abstract
 The overall goal of this proposal is to understand the mechanism by which Rho GTPases,
Rab GTPases, tethering agents, and SNAREs contribute to direct polarized trafficking and growth
at the cell surface. Previous work from our laboratories and others has implicated members of the
Rho/Cdc42, exocyst and Sro7/Tomosyn protein families as highly conserved factors that have
important roles in both polarity and membrane trafficking to the cell surface in systems as diverse
as yeast and neurons. In this proposal, we will make use of new biochemical, genetic, and
structural tools we have developed during the previous funding period to examine the molecular
mechanisms by with Exocyst and Sro7 proteins work together as Rab effectors and vesicle
tethering agents. Importantly we make use of newly identified gain-of-function alleles within the
exocyst and Sro7 to understand the distinct biochemical and structural changes that occur to these
tethering agents as they respond to regulatory and spatial cues.

## Key facts

- **NIH application ID:** 10264083
- **Project number:** 5R01GM054712-24
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **Principal Investigator:** PATRICK J BRENNWALD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $445,663
- **Award type:** 5
- **Project period:** 1998-05-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10264083

## Citation

> US National Institutes of Health, RePORTER application 10264083, Polarized Exocytosis: Rabs, Tethers, and SNAREs (5R01GM054712-24). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10264083. Licensed CC0.

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