# Modulation and functional characterization of protein condensation in chromatin organization

> **NIH NIH U01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $1,086,587

## Abstract

Project Summary
Recent discoveries of the phase-separation abilities of several chromatin regulators have led to novel models
for chromatin organization. Directly testing the causal effects of phase-separation mechanisms requires an ability
to quantitatively tune the phase-separation ability of an endogenous chromatin regulator and simultaneously test
for gene expression and cell fate changes. For this goal, we will develop two tool kits: (1) a phase-dial to enhance
or suppress the phase-separation potential of endogenous proteins in a quantitative and controlled manner, and
(2) a robust and transferrable technological platform to correlate live imaging of phase condensates with the
profiling of gene expression at the single cell level. Utilizing these tools, we will study the regulation and impact
of heterochromatin formation and transcription activation during stem cell differentiation.

## Key facts

- **NIH application ID:** 10264161
- **Project number:** 5U01DK127421-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Bo Huang
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,086,587
- **Award type:** 5
- **Project period:** 2020-09-15 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10264161

## Citation

> US National Institutes of Health, RePORTER application 10264161, Modulation and functional characterization of protein condensation in chromatin organization (5U01DK127421-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10264161. Licensed CC0.

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