MOUSE BIOLOGY SHARED RESOURCE (MBSR) PROJECT SUMMARY/ABSTRACT The Mouse Biology Shared Resource (MBSR) provides unique, sophisticated, and comprehensive resources and services using mutant mice and murine cancer models for basic and translational cancer research for UC Davis Comprehensive Cancer Center (UCDCCC) members. The MBSR has provided research support to cancer researchers at the UCDCCC since its inception. The MBSR offers scientific expertise, customer assistance, technical training, specialized capabilities, unique services, and dedicated infrastructure for mouse embryo manipulation, genome mutagenesis, husbandry, production, phenotyping, and HIPAA-compliant patient derived xenograft (PDX) services. The overall objective of the MBSR is to provide UCDCCC members access to technologies, services, and scientific consultation that assists, facilitates, and supports the application of mouse models in cancer-related research. The MBSR is physically located in dedicated facilities strategically located between the Davis and Sacramento campuses in close proximity to and fully accessible by all UCDCCC members. By ensuring in vivo experimental reproducibility, reliability, cost-effectiveness, and quality control, the MBSR supports and promotes scientific interaction and productivity of UCDCCC researchers using mouse models to study cancer. Oversight and management of the MBSR is provided by the Director, K. C. Kent Lloyd, DVM, PhD, a Professor of Surgery (SOM) and Associate Director for SRs for the UCDCCC, who has extensive experience in mouse mutagenesis, phenotyping, and mouse modeling of disease, including cancer. The interests and productivity of UCDCCC members have generated increasing demand for mutant mouse services. With new genome editing capabilities (e.g., CRISPR/Cas9) and expansion of PDX models, the contributions made by the MBSR to cancer research is projected to continue to expand over the next 5 years. To enhance the scientific interaction and productivity of UCDCCC members, the MBSR will continue to provide cancer-related research support services. The Specific Aims for the MBSR are to: 1) Provide mouse modeling and creation services; 2) Provide mouse husbandry and maintenance services; and 3) Provide mouse genotyping and phenotyping services.