# CSHL Course on Advanced Sequencing Technologies & Bioinformatics Analysis

> **NIH NIH R25** · COLD SPRING HARBOR LABORATORY · 2021 · $80,019

## Abstract

ABSTRACT
COURSE ON ADVANCED SEQUENCING TECHNOLOGIES & BIOINFORMATICS
ANALYSIS
 This Cold Spring Harbor annual short course will train a diverse cohort of biological and
biomedical scientists in the latest sequencing technologies and the downstream bioinformatics
analysis of the large quantities of sequence data generated by these technologies. The
proposed Cold Spring Harbor Laboratory course on Advanced Sequencing Technologies &
Bioinformatics Analysis is to be held November 2021-2025. A broad range of scientific
questions are amenable to exploration through modern approaches in DNA/RNA sequence
analysis, and we therefore anticipate applications from individuals working in areas across the
biological and biomedical sciences, from evolution and development to plant and animal
physiology to cancer and host response to infectious diseases. Students will be instructed in the
detailed operation of several revolutionary sequencing platforms, including library construction
procedures, general data processing, and in-depth data analysis. Common experimental
approaches enabled by massively parallel sequencing technologies will be explored in detail,
including de novo DNA sequencing and assembly of genomes, DNA re-sequencing of known
cancer genes, bulk transcriptome profiling, single-cell transcriptome profiling, small variant
discovery/interpretation and other approaches that are tailored to the student's research areas
of interest. Expanding the bioinformatics component of the course, students will be introduced to
Unix command-line, important file formats, alignment, data visualization, basic scripting in R,
bash and other program languages, cluster job submission and bioinformatics pipeline
development. Guest lecturers contribute to the course and are encouraged to highlight their own
applications of these revolutionary technologies. This specialized training in a residential setting
immediately impacts the progress of the trainees' research, and promotes collaboration and
exchange of ideas between faculty and trainees, and thus represents a significant and valuable
investment towards accelerating genome research. The advanced scope of the course will be
twinned with an innovative educational design, including the broader dissemination of the
course lectures and associated material and problem sets via tailored online learning tools.
Significant outreach to and engagement of underserved research communities and individuals
will be combined with dedicated mentoring and follow-up to help provide ongoing academic
support to and retention of underserved individuals with specific research interests that intersect
with the scope of the course.

## Key facts

- **NIH application ID:** 10270087
- **Project number:** 2R25HG006424-10
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** DAVID J. STEWART
- **Activity code:** R25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $80,019
- **Award type:** 2
- **Project period:** 2012-04-10 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10270087

## Citation

> US National Institutes of Health, RePORTER application 10270087, CSHL Course on Advanced Sequencing Technologies & Bioinformatics Analysis (2R25HG006424-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10270087. Licensed CC0.

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