CORE A - SUMMARY/ABSTRACT: The Vector Immunology Core will directly support all four projects of this program project grant by providing reliable, consistent and standardized immunological assays. It will focus on creating, validating and performing immunological assays to characterize B-cell and T-cell immune responses to AAV, in mice and ferrets. It will also assay booth immune responses to capsid and to transgene product. The core will support all four projects by characterizing B-cell responses by transduction inhibition based NAB assays and ELISAs for capsid binding antibodies and anti-transgene antibodies. It will develop and standardize assays for evaluating ferret B-cell responses. It will additionally characterize T-cell responses by ELISPOT, and cytokine profiling through both ELISA and flow-based cytokine bead assays. Finally, it will develop flow cytometry staining protocols for the evaluation of Ferret immune responses, which are currently not well characterized. Overall the immunology core will provide valuable data, support and resources to all four projects within this program project grant, which will allow for better understanding of the role the immune system in gene and cell therapies.