# Flow Cytometry Shared Resource

> **NIH NIH P30** · COLD SPRING HARBOR LABORATORY · 2021 · $217,476

## Abstract

FLOW CYTOMETRY SHARED RESOURCE – PROJECT SUMMARY/ABSTRACT
Flow Cytometry is essential for cancer research and is widely used to identify, purify and analyze specific cell
populations. The Flow Cytometry Shared Resource has provided high-quality service and access to critical
instrumentation to members of the Cold Spring Harbor Laboratory (CSHL) Cancer Center for over thirty years.
The Shared Resource is led by Faculty Head Douglas Fearon, M.D., and Senior Manager Pamela Moody. Over
the last funding period, this Shared Resource was heavily used to characterize cellular phenotypes using cell
cycle progression, DNA content, apoptosis levels, and resolving multiple cell types through the use of fluorescent
antibodies. The Shared Resource offers four state-of-the-art instruments with up to five different lasers for cell
sorting and analysis. Expert staff is also available for training in the use of equipment, consultation on
experimental design, and assistance with operation of instruments. Access to Flow Cytometry has allowed
development of protocols for single-cell sequencing, the generation and analysis of innovative animal models,
and isolation and characterization of the stem cells and immune cells involved in cancer. The Flow Cytometry
Shared Resource is well used by Cancer Center members; in the last funding period, 31 members (82% of the
membership) used this Shared Resource, accounting for 93% of its use. In the last five years, the Flow Cytometry
Shared Resource supported 61 Cancer Center publications or preprints. Without the Flow Cytometry Shared
Resource, it would be extremely difficult for individual investigators to have access to this sophisticated
instrumentation and these types of services, which are critical to their research programs. In summary, the
Shared Resource provides CSHL Cancer Center members with a complete complement of state-of-the-art
instrumentation and advanced technical support to help accelerate cancer research and discovery.

## Key facts

- **NIH application ID:** 10270220
- **Project number:** 2P30CA045508-34
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** DOUGLAS T FEARON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $217,476
- **Award type:** 2
- **Project period:** 1997-08-01 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10270220

## Citation

> US National Institutes of Health, RePORTER application 10270220, Flow Cytometry Shared Resource (2P30CA045508-34). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10270220. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*