# Organoid Shared Resource

> **NIH NIH P30** · COLD SPRING HARBOR LABORATORY · 2021 · $142,489

## Abstract

ORGANOID SHARED RESOURCE – ABSTRACT
The Organoid Shared Resource was established to provide Cancer Center members with access to the
reagents, equipment, and expertise needed to culture and perform experiments with three-dimensional organoid
models. The organoid technology was introduced to the Cold Spring Harbor Laboratory Cancer Center in 2012
by David Tuveson, with the rationale that these three dimensional cultures recapitulate numerous aspects of
primary tumor biology more faithfully than standard two-dimensional cell lines. There are now 18 Cancer Center
member labs (47% of members) using human and murine organoids in their research, and organoids have been
used in 40 Cancer Center publications or preprints. To support the growing use of organoids, the new Shared
Resource was launched in 2020 with dedicated lab space and staff. The Organoid Shared Resource is being
proposed in this application as a new Cancer Center Shared Resource, led by Faculty Head Camila dos Santos,
Ph.D., and manager Dennis Plenker, Ph.D. The Shared Resource offers members centralized access to
protocols for generating and working with organoid cultures, training in methods, and standardized culture
reagents, as well as equipment for performing high-throughput chemical screens on either two dimensional cell
lines or organoid cultures. Cancer Center members will benefit from access to a biorepository of human organoid
models that have been validated and well characterized. The Shared Resource will be able to offer Cancer
Center members substantial cost-savings due to bulk purchasing of culture reagents, and the development of
protocols to produce media additives in-house. The use of organoids has already fostered several collaborative
Cancer Center projects in line with Cancer Center strategic priorities, including investigating cancer health
disparities in diverse populations, integrating quantitative and wet-bench biologists, and expanding translational
research.

## Key facts

- **NIH application ID:** 10270224
- **Project number:** 2P30CA045508-34
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** Camila dos Santos
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $142,489
- **Award type:** 2
- **Project period:** 1997-08-01 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10270224

## Citation

> US National Institutes of Health, RePORTER application 10270224, Organoid Shared Resource (2P30CA045508-34). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10270224. Licensed CC0.

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