# Normal Aging Lung Cell Atlas (NALCA)

> **NIH NIH U01** · YALE UNIVERSITY · 2021 · $86,005

## Abstract

PROJECT SUMMARY AND SUPPLEMENT TO U01
 The overall objective of the ‘Normal Aging Lung Cell Atlas’ (NALCA), our response to RFA-HL-19-012
(Deciphering the Molecular Landscape of Lung Aging in Humans U01), is to generate a compendium of the
dynamic cell specific changes in mRNA, microRNA, and epigenetic marks that happen during lung aging with a
focus on single cells and the alveolar microenvironment. We plan to use this compendium to generate a dynamic
temporal regulatory model of normal human lung aging. To address this objective, we have assembled a
multidisciplinary group of experts in lung genomics, epigenomics, bioengineering, aging, alveolar development,
systems and computational biology. The premise of this proposal lies in the availability of resources, including
normal human lungs at different ages, aged wild-type and aging relevant genetically modified mice, unique
expertise in high throughput technologies including single cell RNAseq, laser capture microdissection,
methylation profiling, proteomics and tissue engineering, and proven track record in developmental of analytical
approaches that dissect temporal regulatory networks. This objective also largely benefits from synergisms with
other non-overlapping NIH-NHLBI and NIH-NIA projects headed by the coinvestigators on this proposal. We will
address the objectives of this application by the following specific aims: Aim 1: To identify cell and
microenvironment specific changes in coding and non-coding RNAs across human lung aging. Aim 2: To identify
key time points in lung aging using detailed temporal analysis of mouse lung aging. Aim 3: To develop a
comprehensive transcriptional dynamic regulatory multicellular model of lung aging. The completion of these
specific aims will lead to the generation of the Normal Lung Aging Cell Atlas – a resource for the scientific
community that will allow both better understanding of normal lung resident cell aging, as well as the role of aging
related mechanisms in other chronic lung diseases.

## Key facts

- **NIH application ID:** 10275008
- **Project number:** 3U01HL145567-03S1
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** NAFTALI KAMINSKI
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $86,005
- **Award type:** 3
- **Project period:** 2019-01-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10275008

## Citation

> US National Institutes of Health, RePORTER application 10275008, Normal Aging Lung Cell Atlas (NALCA) (3U01HL145567-03S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10275008. Licensed CC0.

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