# THE REGULATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETS) BY ADENOSINE IN MYOCARDIAL ISCHEMIA-REPERFUSION

> **NIH NIH R01** · EMORY UNIVERSITY · 2021 · $78,250

## Abstract

PROJECT SUMMARY
The objective of the proposed research supplement is to understand how donor biologic sex impacts the level
and functionality of surface protein, CD73, on human mesenchymal stem cells (hMSCs). This protein is essential
in the metabolism of adenosine diphosphate (ADP) to anti-inflammatory adenosine (ADO). Some mechanisms
underlying the immunomodulatory capabilities have been well described such as cytokine production, but others
such as purine metabolism are understudied. We aim to understand the difference in purine metabolism by CD73
through the lens of donor sex in order to assess efficacy of hMSCs to mitigate an inflammatory state. hMSCs
are able to mitigate inflammation in many disease states but have shown variability between donors in clinical
trials. Our study will investigate how donor sex plays a role in CD73 expression, CD73 activity level, and cytokine
production, another mechanism of hMSC action in vivo. Together these data will provide critical insight into sex-
dependent differences in hMSC immunomodulation. This work is of importance because it will impact clinical
application of hMSCs by (1) evaluation of cells for specific therapeutic applications, (2) selection of allogenic
donors for broad application, (3) selecting candidates that would benefit from autologous hMSC therapeutics.

## Key facts

- **NIH application ID:** 10275147
- **Project number:** 3R01HL140223-03S1
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** Rebecca Diane Levit
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $78,250
- **Award type:** 3
- **Project period:** 2021-02-23 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10275147

## Citation

> US National Institutes of Health, RePORTER application 10275147, THE REGULATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETS) BY ADENOSINE IN MYOCARDIAL ISCHEMIA-REPERFUSION (3R01HL140223-03S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10275147. Licensed CC0.

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