# SBIR TOPIC 403: HIGHLY ACCURATE SINGLE CELL SEQUENCING WITH SPATIAL RESOLUTION ENABLED BY NANOGENOMICS FORCANCER RESEARCH

> **NIH NIH N01** · NVIGEN, INC. · 2020 · $400,000

## Abstract

The high incidence of mortality in cancer patients is primarily related to drug resistance exhibited by the malignant cells. Through the SBIR project we aim to develop a novel technology that will monitor cancer drug resistance and provide timely screening of alternative drugs to facilitate the most effective course of treatment. This technology will be based on a highly accurate single cell sequencing technology that can interrogate both spatial and temporal resolution using nanogenomics. By integrating a nanopipetting technology that takes miniscule aliquot of single cell samples with NVIGEN highly sensitive magnetic nanoparticle enabled nucleic acid extraction, target enrichment and NGS sample preparation technology, longitudinal genomics of single cells can be measured with spatial and temporal resolution. Our technology enables single cell whole transcriptome analysis, and is orders of magnitude more sensitive than other
methods. In phase I research, we will integrate the nanopipetting and nanoparticle single cell sequencing workflow, making it a useful tool for obtaining single cell sequencing information and assessing drug resistance in tissue samples with spatial resolution. In phase II, we will collaborate with a leading instrumentation and imaging devices company to automate the nanopipetting and nanoparticle library preparation for single cell sequencing with spatial resolution.

## Key facts

- **NIH application ID:** 10280924
- **Project number:** 75N91020C00052-0-9999-1
- **Recipient organization:** NVIGEN, INC.
- **Principal Investigator:** AIHUA FU
- **Activity code:** N01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $400,000
- **Award type:** —
- **Project period:** 2020-09-16 → 2021-06-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10280924

## Citation

> US National Institutes of Health, RePORTER application 10280924, SBIR TOPIC 403: HIGHLY ACCURATE SINGLE CELL SEQUENCING WITH SPATIAL RESOLUTION ENABLED BY NANOGENOMICS FORCANCER RESEARCH (75N91020C00052-0-9999-1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10280924. Licensed CC0.

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