# Proteolytic activity profiling of Alzheimer's dementia

> **NIH NIH F31** · UNIVERSITY OF WASHINGTON · 2021 · $40,805

## Abstract

PROJECT SUMMARY
The incidence of Alzheimer’s disease (AD) and dementia continue to rise, leading to increased burden on our
healthcare system. It is therefore imperative to have a comprehensive understanding of the causative
mechanisms for improved treatment strategies, and potential markers for early detection and intervention for AD.
One causative mechanism of neurodegenerative pathophysiology is aberrant proteolytic processing. A well-
studied example of this is the proteolytic processing of amyloid precursor protein, which can lead to an increase
in amyloid-β peptides, which in turn form aggregates. I aim to investigate the global role of proteolytic processing
in AD by looking for differential proteolytic processing products, and by determining protease activity and
specificity for a couple specific proteases implicated in disease. This will be accomplished by development and
application of several different, but complementary, computational and mass spectrometry proteomics methods
to investigate endogenous protease and substrate abundance profiles and cleavage motif specificity.

## Key facts

- **NIH application ID:** 10295032
- **Project number:** 5F31AG069420-02
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Deanna Lisa Plubell
- **Activity code:** F31 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $40,805
- **Award type:** 5
- **Project period:** 2020-08-16 → 2022-08-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10295032

## Citation

> US National Institutes of Health, RePORTER application 10295032, Proteolytic activity profiling of Alzheimer's dementia (5F31AG069420-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10295032. Licensed CC0.

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