# Defining the programmed proteome rejuvenation underlying gametogenesis

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 2021 · $583,678

## Abstract

ABSTRACT
The integrity of a cell depends on the quality of its components. These components include proteins, which are
responsible for executing most cellular functions, many through organization into stable complexes. A hallmark
of aged cells is the breakdown of protein integrity, or proteostasis, which results from the damage to key proteins
and complexes over time, leading to more accumulated damage, and ultimately cell dysfunction. The specific
proteome components that are most susceptible to damage and that drive its accumulation remain unclear, but
the survival of future generations depends on protection of one cell type—gametes—from inheriting damaged
components from their precursor cell. During gametogenesis in the simple budding yeast, as a precursor cell is
differentiated into gametes, we observe the degradation of many cellular structures and proteins, followed by
their resynthesis and reorganization. This cellular restructuring is associated with an active rejuvenation program
that allows equivalently young gametes to be produced from old or young precursor cells. The mechanisms that
contribute to this natural rejuvenation program are not known, but it can be recapitulated by exogenous
expression of a meiotic transcription factor in aged mitotic cells, suggesting that it is portable.
 Gametogenesis in yeast thus offers the opportunity to watch as the cell shows us what proteins and
complexes it needs to reset and reorganize to ensure cellular youth, and the mechanisms it uses to achieve this.
Of particular interest are proteins of basal or “housekeeping” function, including the ribosome, which are long-
lived in mitotic cells, but degraded and replaced at great energetic cost during gametogenesis. We also observe
reorganization of abundant housekeeping complexes, including the proteasome, during gametogenesis, and
aggregation of some proteins, including superoxide dismutase 1 (Sod1), at the time of their degradation.
Together, these observations suggest that yeast cells remodel their proteome during gametogenesis as a quality
control measure. Here, we propose to identify the key set of cellular components, with a focus on “housekeeping”
proteins, that are reset as gametes are created from precursor cells. We use a proteomic approach to globally
define changes to protein complexes during gamete construction, and specifically determine proteasome
remodeling and activity over time. We investigate the links between aggregation of proteins, including Sod1, and
protein oxidation and degradation. Finally, we identify the specific degradation mechanisms that drive key
aspects of proteome remodeling and test their necessity for gamete rejuvenation and ability to drive lifespan
extension when exogenously activated. This project will build an atlas to reveal the proteins and complexes that
are important enough for young cell identity to warrant the energetic cost of resetting them during gamete
formation, as well as those that may be toxic enou...

## Key facts

- **NIH application ID:** 10298391
- **Project number:** 1R01AG071869-01A1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **Principal Investigator:** Gloria Ann Brar
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $583,678
- **Award type:** 1
- **Project period:** 2021-09-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10298391

## Citation

> US National Institutes of Health, RePORTER application 10298391, Defining the programmed proteome rejuvenation underlying gametogenesis (1R01AG071869-01A1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10298391. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*