# Detecting RORgt Citrullination

> **NIH NIH R03** · BRIGHAM AND WOMEN'S HOSPITAL · 2022 · $85,550

## Abstract

Project Summary
Many proteins are modified after they are produced in cells. Such modification can critically influence the
function of proteins. One such modification is citrullination, which has been shown to regulate the function of
immune cells, including lymphocytes and neutrophils. Abnormal citrullination has been associated with many
human diseases, including infection and autoimmune diseases. Thus, manipulating protein citrullination can be
beneficial in many clinical settings; however, our knowledge of citrullination is still very limited mainly due to
lack of sensitive and specific tool for detecting citrullination. The objectives of this project are to develop a
reagent that can reliably detect the citrullination of a protein named RORgt, which is important for the
differentiation/function of lymphocytes, and to demonstrate the value of this reagent in a proof-of-principle
experiment. The reagent thus generated will be a powerful tool for future studies on the regulatory roles of
citrullination in the function of lymphocytes and very likely will lead to discoveries of novel therapeutic targets of
human diseases.

## Key facts

- **NIH application ID:** 10304200
- **Project number:** 5R03AI155935-02
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** I-CHENG HO
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $85,550
- **Award type:** 5
- **Project period:** 2020-11-17 → 2022-10-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10304200

## Citation

> US National Institutes of Health, RePORTER application 10304200, Detecting RORgt Citrullination (5R03AI155935-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10304200. Licensed CC0.

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