# Extracellular Vesicle Analyses to Develop Aging and Resilience Biomarkers

> **NIH NIH R01** · DUKE UNIVERSITY · 2022 · $646,533

## Abstract

Abstract
Extracellular vesicles (EVs) are membranous particles released from nearly all cell types into all bodily fluids
evaluated to date — including serum and plasma. Depending on tissue of origin, health state and organism
age, they carry a variety of complex cargo consisting of nucleic acids, proteins and metabolites. Although
resilience at a tissue level has largely been attributed to stem cells, recent evidence increasingly points to their
production of EVs as mediators of their remarkable regenerative capacity. The rapid release of small EVs is
induced by physical activity and believed to contribute to the long-term beneficial effects of regular exercise on
muscle metabolism, the cardiovascular system as well as immune modulation. The goal of this project is to
develop biomarkers of aging and resilience through analyses of EVs. Due to their coordinate regulation of
tissue homeostasis and biological processes through intercellular trafficking of microRNA and protein cargo,
EVs are particularly attractive for this project because they can potentially serve as DIRECT biomarkers of
aging and resilience, namely indicators AND mediators of the aging process and response to stressors. We will
use our newly developed 18-channel-high-resolution flow cytometric methodology with validation by
nanoparticle tracking video microscopy, and fluorescence-activated particle sorting that we have established in
the laboratory to evaluate our large existing extensive human sample sets (n=4213 individuals from EPESE,
PALS, STRRIDE, BHS and the Duke 1KP (1000 Patient) cohort) with associated deidentified data and
longitudinal follow-up (6 months to 23 years). We have preliminary data demonstrating an age-related decline
in specific subsets of circulating EVs. We also have identified that a subset of EVs, including some of those
declining with age, are induced with exercise training and predict a beneficial metabolic response to exercise.
These data, together with our collaborators for this project, bring together extensive expertise in aging,
resilience, exercise, and physical performance across the lifespan (ages 18 to 102 years). These resources
and capabilities provide a unique opportunity for us to significantly advance EVs along a biomarker pipeline
and to identify effectors of heathy aging and resilience. For this reason, we are responding to the funding
Opportunity Announcement PA-17-088 that invites applications that employ secondary analysis of existing data
sets or stored biospecimens to address clinically related issues on aging changes influencing health across the
lifespan.

## Key facts

- **NIH application ID:** 10318917
- **Project number:** 5R01AG070146-02
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Virginia Kraus
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $646,533
- **Award type:** 5
- **Project period:** 2021-01-01 → 2025-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10318917

## Citation

> US National Institutes of Health, RePORTER application 10318917, Extracellular Vesicle Analyses to Develop Aging and Resilience Biomarkers (5R01AG070146-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10318917. Licensed CC0.

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