# Molecular Cytogenetics

> **NIH NIH P30** · SLOAN-KETTERING INST CAN RESEARCH · 2022 · $224,604

## Abstract

ABSTRACT
The Molecular Cytogenetics Core provides valuable scientific and technical support to MSK investigators
exploring the role of chromosomal instability in cancer. This is accomplished through appropriate and efficient
experiment design, custom multi-color DNA-FISH probe design and development, data analysis and
interpretation, and support with manuscript/grant preparation.
Currently, the Core provides 2 main categories of specialized service that operate at the single-cell level on a
variety of human and animal model research samples: conventional karyotyping and fluorescence in situ
hybridization (FISH). Conventional karyotyping is routinely performed to confirm cell line identity, screen
targeted mouse ESC clones intended for blastocyst injection, monitor the stability of cultured stem cells over
time, and measure chromosomal instability/heterogeneity as an experimental endpoint. Over the years, the
Core has assembled a broad range of molecular cytogenetics resources (plasmids, BAC clones, individual
whole chromosome painting probes), allowing the Core to provide FISH services at significantly lower cost.
FISH-based assays are routinely employed to map genes or transgene integration sites, detect
aneuploidy/chromosomal instability, co-detect DNA/protein and assess intra-tumoral heterogeneity; tests
include custom probe development, locus-specific FISH, whole-chromosome painting (WCP), spectral
karyotyping (SKY), Q-FISH, and Immuno-FISH. The Core is equipped with 5 modern Fluorescence imaging
systems including 2 with a wide range of applications that can be fully or partially automated. The Core also
provides training in tissue culture, karyotyping, and FISH.
During the past grant period, the services and collaborative work provided by the Molecular Cytogenetics Core
have supported the research of 84 investigators from 8 research program.

## Key facts

- **NIH application ID:** 10319498
- **Project number:** 5P30CA008748-56
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **Principal Investigator:** Gouri Nanjangud
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $224,604
- **Award type:** 5
- **Project period:** 1997-01-20 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10319498

## Citation

> US National Institutes of Health, RePORTER application 10319498, Molecular Cytogenetics (5P30CA008748-56). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10319498. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*