# Molecular Cytology

> **NIH NIH P30** · SLOAN-KETTERING INST CAN RESEARCH · 2022 · $477,125

## Abstract

ABSTRACT
The mission of the Molecular Cytology Core is to deliver cutting-edge, specialized services in support of
research projects at the Center. The methodologies employed by the Core allow for the detection in situ (in
cells, tissue sections, organoids and whole mounts) of biomarkers (proteins, glycoproteins, lipids, nucleic
acids) and molecular processess in normal and pathological conditions. The precise localization of the
biomarkers is achieved by optical imaging of fixed and live samples. Several molecular markers (up to seven)
can be vizualized simultanously or sequentially in the same sample by multiplex staining, using machine-based
protocols. In collaboration with its users, the Core performs comprehensive antibody validations. The Core
trains investigators in basic staining principles and assists them in performing manual experiments. The optical
imaging modalities within the Core allow for wide field imaging and confocal imaging of tissue sections, thick
and cleared tissue samples, cells, live embryos, tumors in live mice, etc. Super-resolution imaging is performed
as well as confocal imaging in the IR range to visualize nanomaterials and drugs. With the newly acquired
module for fluorescence correlation spectroscopy (FCS) imaging, researchers can study the dynamic behavior
of biomolecules. The Core scans user slides with four digital slide scanners, one of which has confocal
capabilities. The scanning generates large volumes of data and serves as a valuable resource for image
analysis. Microscopy staff provides strong support for the users with 2D and 3D image analysis. The Core’s
Atomic Force microscope is heavily used to study molecular interactions with nanometer resolution, to evaluate
the effect of inhibitors on pathologic processes, and to assess stiffness of tissues from normal and cancer-
bearing animals as well as tissues from cancer patients.

## Key facts

- **NIH application ID:** 10319499
- **Project number:** 5P30CA008748-56
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **Principal Investigator:** katia Ognianova manova
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $477,125
- **Award type:** 5
- **Project period:** 1997-01-20 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10319499

## Citation

> US National Institutes of Health, RePORTER application 10319499, Molecular Cytology (5P30CA008748-56). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10319499. Licensed CC0.

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