# MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

> **NIH NIH P20** · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · 2022 · $379,451

## Abstract

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular
localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools
for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the
capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a
range of instrumentation, services, and training for rigorous analysis of structure and function through gene
expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of
molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with
extensive scientific experience in these areas who already provide collaborative assistance to individual
investigators and have significant experience operating core resources. The MACI Core will offer a range of
technical services, and also expert advice in study design, experimental approaches, data analysis and
interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular
analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA-
Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing
(ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue
preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies,
the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and
transmission electron microscopy services. A key service will be to help our PJIs and other investigators to
develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide
training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and
tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training
seminars and project-specific training will expand the knowledge base and capabilities of Geroscience
researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The
MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research
and foster independence, innovation, and creativity of the individual research programs.

## Key facts

- **NIH application ID:** 10320857
- **Project number:** 5P20GM125528-04
- **Recipient organization:** UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
- **Principal Investigator:** WILLARD M FREEMAN
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $379,451
- **Award type:** 5
- **Project period:** 2019-02-01 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10320857

## Citation

> US National Institutes of Health, RePORTER application 10320857, MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE (5P20GM125528-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10320857. Licensed CC0.

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