# Role of CTRP1 in Renal Sodium Handling in Obesity-Related Hypertension

> **NIH NIH K99** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2022 · $90,000

## Abstract

Project Summary
Obesity and its adverse consequences are global public health concerns. Though we have achieved compelling
advances, more effective strategies for managing obesity-associated diseases are still in high demand. Recently,
accumulating evidence from human and animal studies indicate that a novel adipokine, C1q/TNF-related protein
1 (CTRP1), displays multiple beneficial effects on glucose and lipid disorders. This suggests immense potential
for CTRP1 to serve as a novel therapeutic target for obesity-related metabolism disorders. However, the potential
hypertensive effect of CTRP1 limits its application. Therefore, this proposal aims to understand the role of CTRP1
in obesity-related hypertension. Abnormal kidney function and its associated increases in sodium reabsorption
serve as a fundamental mechanism in developing obesity-related hypertension. Nevertheless, no studies have
been carried out to determine the role of CTRP1 in regulating renal sodium reabsorption and blood pressure
during obesity. In preliminary studies, we discovered an undescribed direct regulatory effect of CTRP1 interaction
with (pro)renin receptor (PRR) in controlling ENaC activation in vitro; this may be linked to Nox4-dependent H2O2
production. We presented further evidence that exogenous CTRP1 infusion reduced urinary sodium excretion
and elevated blood pressure accompanied by increased circulating renin-angiotensin-aldosterone system
(RAAS) activity in vivo. These results support the function of CTRP1 in regulating sodium reabsorption and blood
pressure under basal conditions. Emerging evidence from clinical and animal studies reveal a positive correlation
between plasma CTRP1 levels and body mass index or blood pressure. Thus, we hypothesize that CTRP1 plays
a role in determining blood pressure during obesity via stimulating renal sodium reabsorption in two ways: 1)
The local renal mechanism of PRR-dependent ENaC activation; 2) The systemic mechanism of circulating
RAAS-dependent abnormal renal sodium handling. Three specific aims are designed to test this hypothesis. In
Aim 1, we will employ multiple gene targeting techniques to determine the role of CTRP1 in renal sodium handling
and blood pressure during obesity. In Aim 2, we will dissect CTRP1-induced signaling mechanisms involving
coordinated activation of the PRR-dependent pathway and the Nox4/H2O2 pathway in controlling ENaC in
cultured cells. In Aim 3, within adipocyte-specific CTRP1 deletion mice, we will identify the role of adipocyte-
derived CTRP1 in circulating RAAS activation during obesity. Furthermore, we will explore the possibility of
CTRP1 direct affecting renin synthesis or secretion in the juxtaglomerular apparatus by using an isolated
perfused mice kidney model. New information from this proposal would greatly enhance our understanding of
the role of CTRP1 in obesity-related hypertension and lead to novel therapies to manage obesity-related
diseases.

## Key facts

- **NIH application ID:** 10322148
- **Project number:** 5K99HL155850-02
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** Fei Wang
- **Activity code:** K99 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $90,000
- **Award type:** 5
- **Project period:** 2021-01-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10322148

## Citation

> US National Institutes of Health, RePORTER application 10322148, Role of CTRP1 in Renal Sodium Handling in Obesity-Related Hypertension (5K99HL155850-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10322148. Licensed CC0.

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