# In Vivo Modeling of Mitochondrial Complex I Deficiency in Retinal Ganglion Cells > **NIH NIH K08** · DUKE UNIVERSITY · 2022 · $171,109 ## Abstract Candidate: The candidate is a MD/PhD-trained clinician-scientist and board-eligible ophthalmologist currently completing a neuro-ophthalmology fellowship, who will be promoted to Duke Eye Center faculty in July, 2017. His research interest relates to pathobiology and drug discovery in mitochondrial optic neuropathies, a class of blinding disease for which effective therapy does not currently exist. Career Development Plan: The candidate proposes to create a mouse model of retinal ganglion cell (RGC)-specific complex I deficiency, predicted to cause particularly rapid and severe RGC degeneration. The proposed research will allow the candidate to gain experience in animal modeling of human disease, biochemical and histological assays of mitochondrial dysfunction, and retinal electrophysiology. Animal models, reagents, and insights developed in this project will serve as the basis for an R01 proposal to be submitted by the candidate in his final year of K08 support. Specific didactic courses in neurobiology, drug discovery and translation, toxicology, and biostatistics, as well as departmental research seminars and advanced training in responsible conduct of research will be obtained during his K08 tenure, and the candidate will present his findings regularly at national meetings and submit his work for publication. Environment: The candidate’s mentoring team consists of accomplished faculty whose wide range of expertise will be utilized in specific components of the research plan. He will also benefit from informal mentorship and interactions with world-class clinical and research faculty in the Duke Eye Center and from immersion in the dynamic intellectual environment and career development resources available throughout the university. Significant departmental commitment and deep personal investment by the mentoring team will ensure that the candidate is well positioned to transition to an independent R01-funded investigator. Research: Mitochondrial dysfunction frequently results in vision loss from optic neuropathy that reflects the particular sensitivity of RGCs to impaired aerobic metabolism and increased oxidative stress. This application’s central hypotheses are that (1) mitochondria-related RGC degeneration is a cell-autonomous process and (2) RGC metabolism may therefore be manipulated to make these cells less susceptible to mitochondrial insults. Aim 1 will test the first hypothesis by creating a mouse with severe deficiency of mitochondrial complex I specifically in RGCs via conditional knockout of the subunit ndufs4. RGCs in these mice will be assessed for histological, electrophysiological, and metabolic abnormalities. Aim 2 tests the second hypothesis by augmenting Hif-1α signaling with complementary genetic and pharmacologic approaches and assessing whether biasing RGC metabolism toward anaerobic glycolysis makes RGCs resistant to mitochondrial dysfunction and could represent a viable therapeutic strateg... ## Key facts - **NIH application ID:** 10329943 - **Project number:** 5K08EY028610-05 - **Recipient organization:** DUKE UNIVERSITY - **Principal Investigator:** Sidney M Gospe - **Activity code:** K08 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2022 - **Award amount:** $171,109 - **Award type:** 5 - **Project period:** 2018-02-01 → 2023-01-31 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/10329943 ## Citation > US National Institutes of Health, RePORTER application 10329943, In Vivo Modeling of Mitochondrial Complex I Deficiency in Retinal Ganglion Cells (5K08EY028610-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10329943. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*