# Molecular mechanisms of ARF family GTPases

> **NIH NIH R35** · EMORY UNIVERSITY · 2022 · $572,092

## Abstract

Project Summary/Abstract
 Members of the ARF family of regulatory GTPases function as nodes in cell signaling to
coordinate essential cell processes; including membrane traffic, energy metabolism,
ciliogenesis, and the cytoskeleton. I have studied first ARF and later ARF-like (ARL) proteins for
over 30 years, using a combination of biochemical, cell and molecular biological, genetic, and
phylogenetic approaches and propose to continue these studies with a focus on their actions
linked to cilia and centrosomes to allow more detailed mechanistic studies of a subset of five of
the 30 GTPases in this family. We will use genome editing to generate a collection of knockout
cell lines to decipher signaling by the proteins under study. And we will use classical protein
biochemistry to purify and discover novel components in these pathways. We will both study
functions of each of these five GTPases in cells, and link to in vitro biochemical studies of their
enzymology. We will explore the potential for cross-talk or higher level ordering of cell signaling,
and also develop novel, single actions for atypical GTPases as a means of determining shared
or unique mechanisms within the family. A better understanding of these systems will reveal
novel insights into fundamental aspects of cell biology as well as providing potential targets for
intervention to alter the course of human diseases; including but not limited to cancer, heart
disease, neurodegeneration, ciliopathies, retinal degeneration, and deafness.

## Key facts

- **NIH application ID:** 10330783
- **Project number:** 2R35GM122568-06
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** Richard A Kahn
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $572,092
- **Award type:** 2
- **Project period:** 2017-09-15 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10330783

## Citation

> US National Institutes of Health, RePORTER application 10330783, Molecular mechanisms of ARF family GTPases (2R35GM122568-06). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10330783. Licensed CC0.

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