FLOW CYTOMETRY CORE (FCC) – PROJECT SUMMARY The Flow Cytometry Core (FCC), in existence since 1982, is a state-of-the-art Shared Resource that provides University of Virginia Cancer Center (UVACC) investigators access to high-quality, cost-effective flow cytometry services, expertise and technology. The services and expertise offered by the FCC play a key role in the study of many types of cancer, as well as the immune response to cancer. The FCC incorporates a variety of technologies into its services based on the needs of the UVACC Members, thus enhancing the scope and quality of cancer research performed at UVA. The available instrumentation is compatible with a wide array of flow cytometric applications, such as subpopulation identification/quantification, molecular detection, measurement of DNA and RNA content, apoptosis, transcriptional activity, cell viability, membrane potential, extracellular vesicles, cell sorting, and bead-based immunoassays. The FCC staff members are experienced, highly qualified, and SCYM-certified by ASCP. Direct collaborations with UVACC investigators produced novel, published methods and approaches for studying cancer. In addition to providing extensive training in flow cytometry theory and instrumentation, FCC staff provide consultation in experimental design, sample preparation, and data analysis. The facility recently added a site license with Astrolabe Diagnostics, a full- service bioinformatics platform that combines state-of-the-art learning with literature-based analytics to assist UVACC investigators with the complexity of high-dimensional data generated in the flow core. Specialized training classes, web-based training, and consultation are offered for researchers interested in acquiring a better understanding of the principles and techniques employed by this technology and prefer to directly acquire and/or analyze their own samples. Researchers have the option of utilizing the expertise of the FCC staff or performing their own analyses.