PROJECT SUMMARY ANALYTIC MICROSCOPY CORE The overarching goal of the Analytic Microscopy Core (AMC) is to provide scientific and technical expertise in optical microscopy applications that support of high-impact Member studies spanning all facets of cancer research. AMC plays a vital role in the imaging pipeline from tailored study design to the development of novel imaging techniques and the interpretation of resulting data in support of the Members. Further, AMC closely collaborates with other Shared Resources to ensure proper and efficient handoffs of materials and image data. AMC activities are organized into three Specific Aims: Aim 1: To provide experimental design and implementation support for sophisticated microscopy-based studies. Aim 2: To provide access to and assistance on advanced microscopy systems and image analysis platforms. Aim 3: To train and educate Moffitt Members and their staff on the use of state-of-the-art microscopy and image analysis platforms. In support of these aims, AMC has collaborated with 112 Members (91% with peer reviewed funding) across all five CCSG Programs (CBE 26%, CE 14%, MM 30%, HOB 2%, IO 28%), and has performed work on over 100 peer reviewed grants over the last reporting period. During this time, AMC has contributed to 115 publications (25 high impact), which include discoveries in graft versus host disease, mouse melanoma models, immunological features of ovarian cancer, and radiology/pathology correlations in breast tumors. AMC seeks to address the growing needs of the Cancer Center by adding new services and equipment. Based on Member input, during the past funding period new instruments for fluorescence and live cell microscopy were added to increase capacity and provide new technologies. Further, AMC is pursuing other technologies, including high content screening and multiplex fluorescence pathology imaging, to provide these new services to Members. As part of its education programs, AMC provides several training sessions and presentations for existing equipment and services, and also as hosts seminars and demonstrations for emerging microscopy related technology. Future plans for the AMC include services that will provide 1) increased multiplex and whole slide fluorescence imaging capabilities; and 2) high content analysis (HCA) microscopy for rapid, high-throughput, high content and subcellular resolution imaging. Overall, AMC continues to support exceptional research of Moffitt Members by providing cutting-edge microscopy equipment, services and education.