# Genome Engineering Shared Resource

> **NIH NIH P30** · UNIVERSITY OF MINNESOTA · 2022 · $165,907

## Abstract

Genome Engineering Shared Resource Summary
The mission of the Genome Engineering Shared Resource (GESR) is to provide state-of-the-art services in
precision genome engineering of mammalian cell lines to Masonic Cancer Center (MCC) researchers.
Specifically, the GESR is capable of genetically engineering human cell lines tailored to each principal
investigator's (PI's) individual specifications. These services include, but are not limited to 1) single guide RNA
(sgRNA) design; 2) sgRNA validation; 3) sgRNA expression vector construction; 4) design of CRISPR
(Clustered Regularly Interspersed Palindromic Repeats)-associated 9 (Cas9) protein expression vector
reagents; 5) simple, single-locus knockouts for non-essential genes; 6) construction of conditionally null alleles
for essential genes; 7) construction of single-amino-acid knock-in mutations; 8) construction of single-
nucleotide knock-ins; 9) construction of multi-allelic knockout cell lines; and 10) in collaboration with the Mouse
Genetics Laboratory (MGL) Shared Resource, construction of RNA- and protein-based CRISPR/Cas9
reagents for the generation of transgenic mice. These services are essential to the mission of the MCC
because genetic alteration of human cancer cell lines allows PIs the ability to design mechanistic and
therapeutic experiments related to the characterization and investigation of specific cancers. Moreover,
although the demand for genetically modified human cell lines is high, the steps involved in the process of
genome editing are still technically challenging and time consuming and demand resources and a level of
expertise not found in most clinical laboratories. The GESR is capable of providing these services at a very
cost-effective price (well below most commercial sources) and can usually generate the requisite reagent or
cell line as quickly (or more quickly) than most commercial enterprises. The GESR is co-led by Drs. Eric A.
Hendrickson and Branden S. Moriarity and coordinated by Brian Ruis with support from 2 laboratory personnel.
The GESR has been in operation since October 2015.

## Key facts

- **NIH application ID:** 10333243
- **Project number:** 5P30CA077598-24
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **Principal Investigator:** ERIC A HENDRICKSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $165,907
- **Award type:** 5
- **Project period:** 1998-06-01 → 2024-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10333243

## Citation

> US National Institutes of Health, RePORTER application 10333243, Genome Engineering Shared Resource (5P30CA077598-24). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10333243. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*