# Proteostasis at cellular membranes.

> **NIH NIH R35** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2021 · $51,925

## Abstract

Summary (modified parent R35 award)
We investigate E3-ubiquitin ligase regulated protein turnover networks in various membrane
compartments in mammalian cell systems. Thus, enrichment and purification of various
membrane compartments from mammalian cells is essential for understanding the compartment
specific protein turn over. Moreover, our understanding of mechanisms of membrane protein
turnover networks and their phenotypes regulated by UPS remains sketchy. We engineer
mammalian cell systems with various cell-genetic techniques to expose the cell fitness
phenotypes, such as cell proliferation and survival, impacted by membrane anchored E3-
ubiquitin ligases. Therefore, assays for the measurements of accurate cell number and cell size,
in parent and engineered mammalian cells, are indispensable for the cell fitness studies.

## Key facts

- **NIH application ID:** 10339504
- **Project number:** 3R35GM137452-01S1
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Shafi M. Kuchay
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $51,925
- **Award type:** 3
- **Project period:** 2020-08-01 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10339504

## Citation

> US National Institutes of Health, RePORTER application 10339504, Proteostasis at cellular membranes. (3R35GM137452-01S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10339504. Licensed CC0.

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