# Application of single-cell trajectory analysis in single-cell transcriptomics to elucidate the biology of lung pericytes in injury, repair, and regeneration

> **NIH NIH R03** · UNIVERSITY OF WASHINGTON · 2022 · $88,250

## Abstract

ABSTRACT
Pericytes have been implicated in inflammation and fibrosis in a number of organs. Data from
transcriptomic analyses of activated cultured lung pericytes reveal upregulation of multiple
biological pathways including inflammation, angiogenic processes, and cell migration. The goal
of this project is to understand the functional plasticity of lung pericytes throughout injury and in
lung repair. We are interested in how lung pericytes modulate lung repair in lung fibrosis through
angiogenesis and myofibroblast differentiation. In Aim 1, we propose to use state-of-the-art
single cell trajectory analysis on single-cell transcriptomic data to describe the cell fate of lung
pericytes from acute lung injury to fibrosis. In Aim 2, we propose to study selected stromal
subpopulations derived from pericytes and non-pericyte-derived myofibroblasts during fibrosis to
examine their functional differences ex vivo.

## Key facts

- **NIH application ID:** 10340848
- **Project number:** 1R03HL155075-01A1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Chi F Hung
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $88,250
- **Award type:** 1
- **Project period:** 2021-12-15 → 2023-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10340848

## Citation

> US National Institutes of Health, RePORTER application 10340848, Application of single-cell trajectory analysis in single-cell transcriptomics to elucidate the biology of lung pericytes in injury, repair, and regeneration (1R03HL155075-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10340848. Licensed CC0.

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