# Tuning Mitotic Kinesins Through Motor Domain Post Translational Modifications

> **NIH NIH R01** · MAYO CLINIC  JACKSONVILLE · 2022 · $578,697

## Abstract

PROJECT SUMMARY
Many mitotic kinesins play multiple roles in mitosis—each likely requiring distinct mechanical behavior.
This multi-functionality implies that cells need to be able to “fine tune” these motors. We propose that post-
translational modifications (PTMs) of the motor domain (MD) of mitotic kinesins serve this role. These
PTMs are found in many mitotic kinesins and they cluster in structures that form an allosteric pathway
which couples nucleotide and microtubule binding to movement. In this proposal, we will apply a
combination of transient kinetics, single molecule mechanics, and cell biology to examine how MD PTMs
affect three multifunctional kinesins—Kif11, Kif18A, and Kif22. Results of these studies should allow us to
develop an integrated model for how cells modulate their kinesins to meet diverse needs.

## Key facts

- **NIH application ID:** 10348652
- **Project number:** 5R01GM130556-04
- **Recipient organization:** MAYO CLINIC  JACKSONVILLE
- **Principal Investigator:** STEVEN P GROSS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $578,697
- **Award type:** 5
- **Project period:** 2019-03-15 → 2024-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10348652

## Citation

> US National Institutes of Health, RePORTER application 10348652, Tuning Mitotic Kinesins Through Motor Domain Post Translational Modifications (5R01GM130556-04). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10348652. Licensed CC0.

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